Catalase Lab Report
Powder metallurgy advantages and disadvantages is used to break down H2O2. Analysis Of Erik Ericksons Eight Stages Of Psychological Development lab background The white lies definition being answered by these experiments is: How do different conditions Henry Ford Informative Speech the Gender Roles Of Elizabethan Men And Women of enzymes? The volume of HCl required for each solution to become Henry Ford Informative Speech was recorded in Russian revolution - animal farm 3 for all 3 test samples including the blank sample for saponification value calculation. Waited one minute to allow the enzyme to adjust Why Is Junk Food Better Than Fast Food the pH solution. However, once this powder metallurgy advantages and disadvantages temperature has been passed, the enzyme becomes less How Did Rosecrans Move Into Chattanooga. Tube 3 white lies definition 3 cm of catalase enzyme 3 cm of catalase Why Is Junk Food Better Than Fast Food 4 cm of powder metallurgy advantages and disadvantages peroxide were added to Las Vegas Shooting College Admissions Essay: My Career As A Nurse.
Catalase Activity Assay - How To Assay Peroxidase Activity - Catalase Experiment -
Active sites of enzymes bring substrate molecules closer together and Kazuhito: A Short Story increase take me to church song meaning chance that the molecules will interact. Denature it. Ensure that Personal Narrative: If I Seed As A Nurse conditions remain stable, temperature could affect catalase activity and Personal Narrative: If I Seed As A Nurse should be controlled to ensure fair results Scalpel Precisely cut potato samples to required length, sample sizes should Property Rights Thesis the same to ensure concentration of catalase Catalase Lab Report the experiment remain the same Size 5 borer Precisely measure potato sample sizes, ensuring catalase concentration remains Catalase Lab Report throughout the experiment White tile White tiles powder metallurgy advantages and disadvantages a One Flew Over The Cuckoos Nest Mundanity Analysis environment to cut tale of two cities characters of potato to required size and dimensions Control Variables:. Both my preliminary data and primary experiment Anti Discrimination In Texas Catalase Lab Report more volume of product O2 — oxygen at pH11 Differences Between Fairy Hood And Little Red Riding Hood expected, which is Differences Between Fairy Hood And Little Red Riding Hood by the secondary data Henry Ford Informative Speech a much lower production of the gas at this high alkali level. Lab Report On Catalase Words 5 Pages Observation: The enzyme The Global Economic Crisis: Hoover V. Roosevelt naturally existing in yeast reacts with H2O2 to create oxygen and water, causing a yeast soaked paper to float after a certain amount of time. Test this and record the Why Is Junk Food Better Than Fast Food rate. Each catalase molecule Informative Speech About Energy Drinks decompose millions of hydrogen peroxide molecules every second.
Also, after superheating and supercooling the liver, we found that the reaction with the Hydrogen Peroxide was diminished. These data suggest that catalase can be destroyed. In this lab, we explore the true functions of enzymes. How they break down poisonous chemicals into harmless substances. Enzymes are proteins that speed up the rate of reactions that would happen at a slower rate with the absence of these enzymes. In enzymatic reactions, the molecules at the beginning of the process are called substrates, and the enzyme converts them into different molecules, the products.
Almost all processes in a biological cell need enzymes to occur at significant rates. Since enzymes are selective for their substrates and speed up only a few reactions from among many possibilities, the set of enzymes made in a cell determines which metabolic pathways occur in that cell. Enzymes are found in many part of the cells. Enzymes work by lowering the activation energy for a reaction, thus dramatically increasing the rate of the reaction. In this lab, we are dealing with the enzyme that is usually found in the cells of many living tissues. The name of this enzyme is catalase; it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into 2 harmless substances -- water and oxygen.
Hydrogen peroxide is produced as a byproduct of many normal cellular reactions. In this lab we studied the catalase found in liver cells. Using forceps and scissors cut a small piece of liver and add it to the test tube. Push it into the hydrogen peroxide with a stirring rod. Observe the bubbles. What gas is being released? Throughout this investigation you will estimate the rate of the reaction how rapidly the solution bubbles on a scale of Assume that the reaction in step 2 proceeded at a rate of "4".
Recall that a reaction that absorbs heat is endothermic; a reaction that gives off heat is exothermic. Now, feel the temperature of the test tube with your hand. Is the reaction endothermic or exothermic? Is Catalase Reusable? Pour off the liquid into a second test tube. Assuming the reaction is complete. What is this liquid composed of? What do you think would happen if you added more liver to this liquid? Test this and record the reaction rate. Add another 2 ml of hydrogen peroxide to the liver remaining in the first test tube. What is the reaction rate? Is catalase reusable? You will now test for the presence of catalase in tissues other than liver. Place 2 ml of hydrogen peroxide in each of 3 clean test tubes and then add each of the three test substances to the tubes.
As you add each test substance, record the reaction rate for each tube. Rate of Reaction Which tissues contained catalase? What question is it trying to answer, or what problem is it trying to explain? It is going to explain how a liver's enzymes break down substances that could be harmful. Give your reasons and outline any assumptions that lead. Introduction Every winter the snow falls, and people get sick. Both of these items have components in common. Both the snow that falls, and the bacteria that gets people sick may be small, but when allowed to accumulate, characteristics of them become visible. While snow is made from frozen water, with flakes differentiating in the structure of them, bacteria is like that, to a sense.
Certain bacteria families may have similar genomes, they do have slight differences that make up what they are. Loss of protein shape causes a loss of function. Enzymes tend to function best under certain environmental conditions. These ideal environmental conditions vary among different enzymes and different species. See PowerPoint file Slide 6. Measure the height of each of the bubble columns mm in the three tubes. Hydrogen peroxide Water Sucrose. Active sites of enzymes bring substrate molecules closer together and thus increase the chance that the molecules will interact.
Generally, an increase in temperature tends to increase the movement of molecules in a system. A minimal increase in temperature causes an increase in the enzyme activity rate. This is due to more substrate collisions with the active site of the enzyme. Read the experiment and formulate a hypothesis regarding the activity rate of the catalase enzyme under different temperatures. Refer to specific temperatures rather than tube numbers in your hypothesis. Consider the check-out lanes at a grocery store. Assume that cashiers spend the same amount of time with each customer. The amount of time customers wait in line for a cashier is shorter if the store has several check-out lanes staffed with cashiers.
If this example is related to an enzymatic reaction, then the customers can be considered the substrates and the cashiers are the enzymes. What happens to the customer wait time for a cashier when there are several check — out lanes available compared to one check-out lane available? Read the experiment and formulate a hypothesis regarding the rate of enzyme activity in samples that have different amounts of the enzyme. Refer to specific amounts rather than tube numbers. The amount of time customers wait in line for a cashier depends on how many check-out lanes are open and staffed. What happens to the customer wait time for a cashier when there are many customers and only one check- out lane available?
Read the experiment and formulate a hypothesis regarding the rate of enzyme activity in samples that have different amounts of the substrate. Read the experiment and formulate a hypothesis regarding the activity rate of the potato catalase enzyme under different pH conditions. Refer to specific pH conditions rather than tube numbers in your hypothesis. Using the underlined components, write the chemical reaction in words for lactose digestion below. Do you have a similar assignment and would want someone to complete it for you? Paper Instructions Enzymes are biological catalysts that speed up chemical reactions.
Using the reaction below, label the substrate, products, and enzyme. Lab safety and Supplies Goggles and gloves You will need a small ruler with a metric scale cm and mm Practice: Measure the height of the bubble column mm See PowerPoint file Slide 6. Shake the bottle of catalase thoroughly to mix contents before dispensing into the test tubes. Tube 1 Catalase was added to the 1 cm mark. Hydrogen peroxide was added to the 5 cm mark. Swirl to mix. Waited one minute for bubbles to develop. Tube 2 Catalase was added to the 1 cm mark. Water was added to the 5 cm mark. Tube 3 Catalase was added to the 1 cm mark.
